Hexane ACS Reagent Grade

Product Overview

Hexane ACS Reagent Grade (synonyms: n-Hexane, Hexane 99%) is a clear, colorless liquid hydrocarbon with the chemical formula C₆H₁₄. It is widely utilized as a non-polar solvent in analytical and preparative workflows due to its low boiling point, high volatility, and favorable solvency for non-polar organics. Complying with ACS Reagent Grade specifications and USP-NF Monograph expectations, this hexane is curated to deliver consistent batch-to-batch performance for sensitive applications, including chromatographic separations and extraction protocols. Typical assay purity is 99.8% with a possible range of 99–100.5%, validated via Gas Chromatography (GC) with FID, ensuring reliable solvent quality for demanding lab environments. The product also features low acidity and controlled impurity levels, supporting stable baselines in gradient HPLC methods and reproducible extraction efficiencies. The material is stored under proper conditions to minimize moisture uptake and maintain chromatographic reproducibility across lots.

Key Properties

• CAS: 110-54-3
• Appearance: Clear, colorless liquid
• Odor: Characteristic petroleum-like odor
• Boiling Point (°C): 68.7 - 69.1
• Freezing Point (°C): -95
• Solubility: Insoluble in water, soluble in organic solvents
• Flash Point (°C): -25
• Vapor Pressure (20°C): 160 mmHg
• Density (25°C): 0.655 g/mL
• Specific Gravity (20°C): 0.656 (typical)
• Molecular Weight: 86.18 g/mol
• Molecular Formula: C₆H₁₄
• Refractive Index (20°C): 1.375

Common Applications

• HPLC Mobile Phase: Commonly used as a mobile phase component in reversed-phase chromatography for separating organic compounds.
• Extraction Solvent: Utilized in the extraction of active pharmaceutical ingredients from plant materials.
• Cleaning Agent: Effective for cleaning and degreasing metal parts in manufacturing processes.
• Solvent for Reactions: Serves as a solvent in various organic reactions due to its non-polar nature.
• Standard Reference Material: Used as a standard in calibrating analytical instruments in research settings.

Safety Precautions

Handle and store Hexane ACS Reagent Grade in a cool, well-ventilated area away from heat sources, open flames, and oxidizing agents. Use compatible containers (HDPE or glass) with tightly closed caps to minimize evaporation and moisture ingress. Employ appropriate PPE, including chemical-resistant gloves, splash goggles, and lab coats, and ensure adequate ventilation or fume hood use during handling and transfer to prevent inhalation of vapors and reduce exposure risk. Maintain away from ignition sources and follow local regulatory requirements for flammable liquids.

For spill response, evacuate non-essential personnel and ventilate the area; absorb with inert absorbent material and collect in flammable waste containers per SDS guidelines. In case of skin or eye contact, flush with water for an extended period and seek medical attention if irritation persists. If inhaled, move to fresh air and seek medical evaluation if dizziness or respiratory symptoms occur. Store away from incompatible substances and consult the SDS prior to use for complete hazard and first-aid details.

Benefits

✔ High Purity ACS Reagent Grade – Meets ACS specifications and USP-NF monographs, providing consistent performance for analytical methods and extraction protocols.
✔ Low Impurity Profile – Controlled acidity (≤0.0003 meq/g) and low aldehyde content support stable baselines in HPLC and reliable solvent quality across batches.