60/40 Methanol/DI Water Solution ACS Grade

Product Overview

An ACS Grade 60/40 Methanol/DI Water Solution is a precisely formulated binary solvent system designed for analytical and preparative workflows. This 60/40 v/v mixture of methanol (CH₃OH) and deionized water (H₂O) provides a balanced polarity suitable for reversed-phase chromatographic separations and UV-transparent performance below 205 nm, making it ideal for HPLC mobile phases, spectroscopy, and critical dilution steps. The solution is prepared to ACS Reagent Grade standards with strict control of water content, acidity, and trace impurities to ensure consistent chromatographic baseline stability and reproducibility across batches. Typical assay specification ranges from 99% to 100.5% with exceptional low aldehyde and metal impurity levels, supporting high-purity requirements in pharmaceutical and chemical synthesis environments.

This solvent system is optimized for UV-Vis transparency, low acidity, and minimal color interference, enabling reliable detection in sensitive analytical methods. Its compatibility with a wide range of organic solvents and miscibility with water facilitates robust sample preparation, extraction, and reaction media. The product is supplied with comprehensive COA data and meets both ACS and USP-NF monographs, ensuring traceability, quality, and regulatory alignment for routine laboratory use.

Key Properties

• Property Name: Chemical Formula CH₃OH/H₂O (60/40 v/v)
• Chemical Formula: CH₃OH and H₂O (60/40 v/v)
• Molecular Weight: 32.04 g/mol
• Boiling Point: 64.7°C
• Specific Gravity: 0.787 g/mL at 25°C
• Density (25°C): 0.787 g/mL
• Vapor Pressure (20°C): 97 mmHg
• Refractive Index (20°C): 1.3288
• Viscosity (25°C): 0.54 cP
• Flash Point (Celsius): 11°C
• Appearance: Clear, colorless liquid
• odor: Slightly sweet odor
• Molecular Weight (expanded): 32.04 g/mol
• Partition Coefficient (logP): –0.78 (approximate for methanol/water mix)
• Decomposition Temp: Not applicable (decomposes before melting; standard handling at 20°C-25°C)

Common Applications

• HPLC Mobile Phase: Analytical Chemistry — widely used as a mobile phase component in reversed-phase HPLC for separating organic compounds with robust UV transparency and reproducible retention times.
• Spectroscopy Solvent: Research Laboratory — serves as a baseline solvent for UV-Vis measurements due to a low UV cutoff and minimal background interferences.
• Extraction Medium: Pharmaceutical Manufacturing — employed in liquid-liquid extraction processes for isolating pharmaceutical intermediates with controlled polarity.
• Reagent for Chemical Reactions: Chemical Synthesis — functions as a solvent and participating reagent in organic synthesis and reaction media optimization.
• Cleaning Agent: Industrial Cleaning — used to remove residues and contaminants from laboratory equipment with compatibility across materials.
• Calibration and Sample Dilution: Analytical workflows — provides a stable diluent with low impurity burden for accurate quantification.
• UV Transparent Solvent for Assays: Analytical Methods — suitable for UV-based assay development requiring transparency below 205 nm.

Safety Precautions

Handle and store the methanol/water solution in well-ventilated areas, away from heat sources and oxidizers. Use HDPE or glass containers and ensure tightly closed closures to minimize vapor release and moisture ingress. Wear appropriate PPE, including chemical-resistant gloves, safety goggles, and lab coats. Maintain proper ventilation and avoid inhalation of vapors; use fume hoods during handling and transfer operations. Adhere to standard storage conditions (cool, dry place) and prevent exposure to incompatible materials to reduce the risk of pressure buildup or contamination.

Exercise caution with exposure routes and emergency procedures: methanol is toxic if swallowed, inhaled, or absorbed dermally, and exposure should be minimized. In the event of exposure, follow SDS-guided first aid: rinse affected eyes/skin with water; seek medical attention for inhalation or ingestion. In the event of a spill, evacuate the area, ventilate, contain with inert absorbent material, and dispose of per local regulations. Refer to the safety data sheet for complete hazard statements, including H225 (flammable liquid) and H301/H311/H331 (toxic effects), and ensure UN 1230 Methanol packaging for transport.

Benefits

✔ ACS/USP-Compliant – Meets ACS Reagent Grade and USP-NF monograph requirements for reliable, regulatory-aligned use in analytical and pharmaceutical workflows.
✔ Consistent Polarity and UV Transparency – The fixed 60/40 ratio provides predictable chromatographic behavior and low UV interference in detections below 205 nm.
✔ Low Impurity Profile – Controlled trace metals and impurity levels minimize baseline drift and method variability in sensitive methods.