60/40 Methanol/ DI Water Solution

Product Overview

A precisely formulated 60/40 v/v mixture of methanol (CH₃OH) and deionized water (H₂O) designed for robust analytical performance in demanding laboratory applications. This binary solvent system provides optimized polarity for reversed-phase chromatography, supporting reliable separation of a wide range of organic compounds while delivering excellent UV transparency below 205 nm. The solution is produced to ACS Reagent Grade and USP-NF monograph standards, ensuring consistent quality and traceability across batches. Its controlled water content and low acidity minimize baseline drift in HPLC and spectroscopic methods, enabling reproducible results in trace-level analyses.

Its low UV cutoff, combined with stable density and refractive index at 20°C, makes it a versatile baseline solvent for UV-Vis spectroscopy and chromatographic workflows. The product is stored under inert conditions to preserve UV transparency and minimize water uptake from the atmosphere, preserving solvent performance in critical analytical methods. This solution is suitable for a range of applications from mobile phase preparation in HPLC to serving as a clean extraction medium in pharmaceutical processes, with a clear, colorless appearance and a slight, characteristic alcoholic odor.

Key Properties

• Appearance: Clear, colorless liquid
• Chemical Formula: CH₃OH + H₂O (60/40 v/v)
• Molecular Weight: 32.04 g/mol
• Boiling Point: 64.7°C
• Specific Gravity: 0.791 (20°C; typical)
• pH: 6.5–7.5 (typical 7) in a 1:10 solvent:water mixture
• Flash Point: 11°C
• Vapor Pressure (20°C): ~440 mmHg
• Viscosity (25°C): 0.54–0.60 cP (typical 0.60 cP)
• Refractive Index (20°C): 1.3288
• Water Content: 40% (Karl Fischer method)
• Residue After Ignition: ≤0.01% w/w (typical 0.005%)
• Chloride (Cl⁻): ≤1 ppm (typical 0.2 ppm)
• Nitrate/Nitrite (NO₃⁻/NO₂⁻): ≤1 ppm (typical 0.2 ppm)
• Heavy Metals (as Pb): ≤0.2 ppm (typical 0.05 ppm)
• pH Stability: Low acidity (≤0.02 meq/g; typical 0.01 meq/g)

Common Applications

• HPLC Mobile Phase: Used as a primary or co-solvent component in reversed-phase chromatography to achieve consistent retention and peak shape for organic analytes.
• Spectroscopy Solvent: Suitable as a baseline solvent for UV-Vis measurements due to low UV cutoff and high optical clarity.
• Extraction Medium: Employed in liquid-liquid extraction workflows for pharmaceutical intermediates and organic compounds, balancing polarity and miscibility.
• Cleaning Agent: Effective for glassware and instrument cleaning where methanol content aids solubilization of residues while preserving equipment integrity.
• Reagent in Chemical Synthesis: Acts as a solvent and participating medium in various synthetic routes requiring a controlled polarity solvent.

Safety Precautions

Handle with appropriate PPE (gloves, goggles, lab coat) in a well-ventilated area. Store in a cool, dry, well-ventilated location away from heat sources and direct sunlight in HDPE or glass containers. Keep containers closed when not in use and minimize exposure to air to reduce moisture uptake and maintain UV transparency. Follow standard operating procedures for flammable solvents and ensure compatible storage with oxidizers away from ignition sources.

In case of contact with skin or eyes, rinse thoroughly with water and seek medical attention if irritation persists. If inhaled or ingested, move to fresh air and consult medical personnel immediately. This product is highly flammable; use explosion-proof equipment and avoid open flames. In case of a spill, ventilate the area, remove ignition sources, and absorb with appropriate inert material for safe disposal according to local regulations. Refer to the Safety Data Sheet (SDS) for comprehensive handling, exposure controls, and emergency procedures.

Benefits

✔ Controlled binary solvent system – Delivers predictable polarity for reproducible separations in analytical workflows.
✔ UV-transparent and low absorbance – Supports accurate measurements below 205 nm in HPLC and spectroscopy.